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. Author manuscript; available in PMC: 2008 Jan 2.
Published in final edited form as: Mol Cell Endocrinol. 2006 Oct 23;260-262:83–92. doi: 10.1016/j.mce.2006.06.012

Figure 5. Ability of hFSH and hFSHδ4 to stimulate cyclic AMP accumulation.

Figure 5

CHO cells (50,000) that express the hFSHR were incubated with hFSH or hFSHδ4 in 60 μl of Krebs-Ringer solution buffered with 20 mM HEPES (pH 7.4) for 20 min at 37°C. The incubation tubes were heated at 75°C for one minute to stop the reaction and cause the release of all cyclic AMP. The cyclic AMP was measured using an RIA employing a rabbit antiserum and a radioiodinated cyclic AMP derivative prepared as described (Brooker, Harper, Terasaki, & Moylan 1979). The values are means of triplicates and the vertical bars extend to the limits of the SEM. There was no significant difference between either treatment in any of the three independent assays performed.