Immunoblotting analyses with the indicated antibodies. A, FLAG-tagged WT β-catenin or pFLAG was co-transfected with or without AKT1 DD or AKT1 AAA into 293T cells, followed by immunoprecipitation with anti-14-3-3ζ antibody. B, FLAG-tagged WT β-catenin, β-catenin S552A, or β-catenin S552D was transfected into 293T cells, followed by immunoprecipitation with anti-14-3-3ζ antibody.