Figure 5.

Overexpression of Mad2 is not required for tumor maintenance. (A) Axial MR images of the abdomen and the lungs of bitransgenic mice on normal diet (Mad2 on) and after 2 weeks on doxycycline food (Mad2 off) showing the presence of tumors (white arrows). (B) H&E (top) and Ki67 staining (bottom) of a hepatoma in a bitransgenic TetO-Mad2/CMV-tTA mouse untreated (Mad2 on) (left) and a mouse fed doxycycline for 15 weeks after confirming the presence of a tumor by MRI. (C) QRT-PCR confirming the downregulation of Mad2 in the tumor. Black bar 100μm, red bar 20μm and blue bar 1cm.