Figure 7.

Mitotic progression of in vitro stimulated lymphocytes. (A) Percentage of lymphocytes positive for MPM2 as a marker of mitosis. (B) Western blot analysis of in vitro stimulated lymphocytes isolated from spleen of non transgenic mice and TetO-Mad2/CMV-rtTA mice in the presence of doxycycline showing stabilization of cyclin B1 in the Mad2 overexpressing cells as compared to the non transgenic cells (C) Western blot analysis of in vitro stimulated lymphocytes showing stabilization of securin in the TetO-Mad2/CMV-rtTA cells compared to the non transgenic control.