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Purification by isoelectric focusing of cytosolic 12(S)-HETE binding complex. LLC cells were incubated with 0.1 nM 12(S)-[3H]HETE. Cytosol was prepared and subjected to isoelectric focusing in a Rotofor system. Fractions were withdrawn and the radioactivity (○) and pH (□) were measured. In the presence of a thousandfold excess of unlabeled 12(S)-HETE, the peak in fraction 9 was undetectable.
