Figure 3.

Ligand-dependent interaction between 50-kDa 12(S)-HETE binding protein and SRC-1. Cytosol from LLC cells was treated with 10 mM ATP and chromatographed on Superdex 200. The fraction containing the 50-kDa 12(S)-HETE binding protein was collected and incubated with (○) or without (■) 1 nM 12(S)-HETE for 1 h at 4°C. This incubation was followed by an incubation with 6.5 nCi [³⁵S]methionine-labeled SRC-1 for 1 h at 4°C. The samples were rechromatographed on Superdex 200, and fractions were collected for radioactivity measurements.