Table 1.
List of target proteins that were tested by using in vitro phosphorylation assays
| Name | Genome no. | Predicted/actual kDa | Fold change of spot vol(overexpressed/knockout) | Coverage, % | Spot, no. | Phosphorylated by SnRK2.8 |
|---|---|---|---|---|---|---|
| 14-3-3χ | At4g09000 | 30.0/33.8 | 52.6 | 27 | 503 | Yes |
| 14-3-3κ | At5g65430 | 27.8/29.8 | Present/absent | 33 | 538 | Yes |
| 14-3-3ψ | At5g38480 | 28.7/26.4 | 10.0 | 14 | 560 | Yes |
| Glyoxalase I | At1g11840 | 31.9/35.2 | Present/absent | 44 | 484 | Yes |
| Adenosine kinase I | At3g09820 | 38.2/36.8 | Present/absent | 15 | 452 | Yes |
| Ribose 5-phosphate isomerase | At3g04790 | 29.4/27.5 | Present /absent | 28 | 623 | Yes |
| 60S acidic ribosomal protein P2 | At2g27720 | 11.4/10.6 | Present/absent | 39 | 813 | Yes |
| OTU-like cysteine protease | At1g50670 | 23.6/24.5 | Present/absent | 13 | 544 | No |
| Tubulin α-2/α-4 chain | At1g04820 | 50.1/54.8 | 2.75 | 36 | 329 | No |
| PP2A-3 protein phosphatase | At2g42500 | 36.4/36.8 | Present/absent | 13 | 452 | Unable to produce protein |
| Heat-shock protein 70 | At3g09440 | 71.5/75.2 | Present/absent | 19 | 222 | Unable to produce protein |