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. 2007 Mar 29;104(15):6176–6181. doi: 10.1073/pnas.0609915104

Fig. 3.

Fig. 3.

Reconstitution of RNR complexes using purified proteins. (A) Coexpression and purification of NrdA-a and NrdA-b by dATP-Sepharose and Superose6 gel-filtration chromatography. A schematic of the pNrdA-a/NrdA-b expression plasmid is shown, with T7 RNA polymerase promoters (T7) indicated by right-facing arrows and transcriptional termination signals indicated by stars. Shown are 12% SDS/PAGE gels with aliquots of each purification step. Fractions from wash or elution steps are indicated above the gel. M, molecular mass standards (in kilodaltons); U, uninduced extract; I, induced extract; C, crude lysate; S, soluble fraction; L, column load; FT, column flowthrough. (B) Coexpression and purification of NrdA-a, NrdB, and NrdA-b, labeled as in A.