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Detection of open CE breaks in WT and ATM-deficient DP thymocytes. Genomic DNA was isolated from sorted DP thymocytes of Atm−/− mice and control Atm+/+ littermates, as described in Fig. 5 and Materials and Methods, and ligation-mediated PCR was performed to measure open CE breaks. Results shown are PCR amplifications from 3-fold dilutions of ligation product.
