Fig. 2.

Kinetics of polymerization and depolymerization of actin is largely unchanged by CaMKII. Effect of CaMKII on the kinetics of polymerization (a) and depolymerization (b) was examined by using pyrene-labeled actin. (a) G-actin (2 μM, 5% pyrene-labeled) was polymerized with the addition of actin polymerization buffer in the presence (gray line) or absence (black line) of CaMKIIβ (2 μM as monomer). (b) Actin filament disassembly was induced in the absence (black line) or presence of 2 μM CaMKIIβ (gray line) by dilution of preformed 5% pyrene-labeled actin filaments to a final concentration of 0.2 μM. Error bars indicate SEM of three experiments, shown every 1 min.