Fig. 1.

Immunoprecipitation of RyR2 by PC2. (a) Expression of RyR2 and PC2 in canine and mouse heart. Lysates from canine and mouse heart were resolved on SDS/PAGE and immunoblotted (IB) with anti-RyR, anti-PC2 (YCC2), or anti-calnexin antibodies. Lysate, in lane 1, is from canine heart. Calnexin was used as the loading control in these experiments. The upper two panels are from the same blot, and the lower two panels are from the same blot. (b) Coimmunoprecipitation of RyR2 by PC2. Lysate isolated from mouse heart was subjected to immunoprecipitation (IP) with YCC2. Samples were blotted with antibodies to RyR2, PC2 (YCC2), and SERCA-2a. In these experiments, SERCA-2a was used as a negative control.