Figure 3.

CCN5 expression pattern in rat carotid artery balloon injury model. The common carotid artery of a male rat was subjected to a de-endothelializing injury with an inflated balloon catheter inserted. Rats were sacrificed just before injury and at 2 and14 days after injury. The injured segment of the artery was dissected free of surrounding tissue, fixed, and imbedded in paraffin. Sections were cut, dewaxed, and stained for CCN5 using the anti-CCN5 rabbit polyclonal antibody described earlier. Texas Red-conjugated donkey anti-rabbit antibody was used as secondary. A: Uninjured carotid artery. B: Day 2 after injury. C: Day 14 after injury. D: Uninjured carotid artery, preincubated with blocking peptide. E: Day 14 after injury, preincubated with blocking peptide. F: Day 14 after injury, secondary antibody alone.