Table 1.
Primer Sequences for the Amplification of the FAS Gene on Genomic DNA Isolated from 30 to 100 Microdissected RS or Infiltrating Cells
| Exon | External primer set | Nested primer set 29 | PCR (bp) | ||
|---|---|---|---|---|---|
| Forward primer 5′—3′ | Reverse primer 5′—3′ | Forward primer 5′—3′ | Reverse primer 5′—3′ | ||
| 1 | (40 GC)TCAGTACGGAGTTGGGGAAGC | GCCTATCCCCGGGACTAAGAC | 136 | ||
| 2 | (40 GC)ATCAATAAAATTCTCTTCATG | GACTTTCACTGTAATCTCTGG | 239 | ||
| 3 | AAACACTTGCTCCTTTTTTCC | (40 GC)TGAAATTCCAAGATTGGCC | 213 | ||
| 4 | TCCAAACTGATTTTCTAGGC | (40 GC)TCTAGTGTTTTAATCAGAGAAAGAC | 162 | ||
| 5 | (40 GC)CCAGGCTTTTGAATTTCTCC | GGGAAAGGAGGATATAACCG | 133 | ||
| 6 | TAATATGCCAATGTTCCAACC | (40 GC)CCCCAAGTTATTTCAATCTG | 173 | ||
| 7 | GGCCACTTTTAAGTTTCACTG | AGCAAGACTCCATCTCAAAC | CATGCATTCTACAAGGCTGAG | (40 GC)AGGAAGTAACAAAAAGCCAA | 254 |
| 8 | GCAACTGATTGTACTTCTTTC | TCATACGCTTCTTTCTTTCC | TCTCTGCTTCCATTTTTTGC | (40 GC)TTTACTCTGAAATTGGCCTA | 160 |
| 9.1 | TATTTCTATTTTTCAGATG | CAAACACTAATTGCATATACTC | (40 GC)TATTTTCTATTTTTCAGATG | TCATACGCTTCTTTCTTTCC | 253 |
| 9.2 | TATTTCTATTTTTCAGATG | CAAACACTAATTGCATATACTC | GTTCAACTGCTTCGTAATTG | (40 GC)GAACTGAATTTGTTGTTTT | 249 |
40 GC; GC clamp consisting of 40 nucleotides; 29 bp, base pair.