Figure 2.

Restriction cuts sorted by MwoI enzyme. Analysis refers to patient 1. Top: Wild-type (left) and patient (right) sequences, spanning nucleotides 1073 to 1558/1557 of exons 10 to 12, and the positions of the gcnnnnn/nngc cuts by the MwoI restriction enzyme. Bottom: Control and patient fragments sorted by MwoI cuts resolved by ethidium-bromide 2.5% agarose gel electrophoresis and ultraviolet transillumination. Underlined, physiological cut sites by the MwoI enzyme; bold, additional cut-site in patient; g (bold and italics), G deleted in patient.