Figure 3.

Western blot analysis of a3 and TIRC7 proteins. Proteins were extracted from peripheral blood mononuclear cells of control and patient 1, as described in Materials and Methods, and resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Immunoblotting was performed using an antiserum against the C-terminal peptide of the a3 V-ATPase subunit, also recognizing the TIRC7 splice variant of the ATP6i gene (middle). Long-term film exposition allowed detection in these cells also of the modestly expressed a3 protein (top). Bottom: The constitutive protein actin used as an internal control.