Table 2.

Primers and PCR Conditions for DNA Sequencing

Primers	Primer sequences	Genomic position	No. of bases	AT	Product size	Exons
116KDa-A Forward	5′-GTG CAC AGG TGC CCG TGG TT-3′	2221–2240	20	60°C	720 bp	2–3
116KDa-A Reverse	5′-CCC AGA CTC TTC CTT TCA GA-3′	2940–2921	20
116KDa-B Forward	5′-CTG GTG GCC GAT GGA GTT TG-3′	3578–3597	20	60°C	620 bp	4–5
116KDa B Reverse	5′-AGT TCC GGG CCT GAA GGA GG-3′	4197–4178	20
116KDa C Forward	5′-GGC CAG TGT GCC CAA TTG CC-3′	4281–4300	20	60°C	729 bp	6–8
116KDa C Reverse	5′-ACC TCC TGC ACC CAC CTC CG-3′	5009–4990	20
116KDa D Forward	5′-CGG AGG TGG GTG CAG GAG GT-3′	4990–5009	20	65°C	486 bp	9
116KDa D Reverse	5′-AGG CAA AGC CCA GGT GCA GG-3′	5475–5456	20
116KDa E Forward	5′-AGG GCA GAG CAG GGC TGA TC-3′	5826–5845	20	60°C	374 bp	10
116KDa E Reverse	5′-TCA GGC TCA CAC CCT CCC AG-3′	6199–6180	20
116KDa-F Forward	5′-GGG TTC TTG ACT GCA GGC CA-3′	8357–8376	20	60°C	676 bp	11–13
116KDa F Reverse	5′-TCA CCA CCC ACG GAC ACT CC-3′	9032–9013	20
116KDa G1 Forward	5′-GCT GGC CCA TCT GCG CTC TG-3′	9740–9759	20	65°C	717 bp	14
116KDa G1 Reverse	5′-TGA GCT CCG GCA GCG TCT CC-3′	10156–10137	20
116KDa G Forward	5′-AGA TTT GGA GCC TGG CTG CC-3′	9903–9922	20	65°C	498 bp	15
116KDa G Reverse	5′-TCT TGC AGC TCC CAG TGG CC-3′	10400–10381	20
116KDa H Forward	5′-AGG TGT GCA CAG CAG GGA CG-3′	10642–10661	20	60°C	549 bp	16–18
116KDa H Reverse	5′-AGA GAA GCA ACC CGC CCA GC-3′	11358–11339	20
116KDa I Forward	5′-GTG CAG GGA GGG CTT CAG GC-3′	11421–11440	20	65°C	417 bp	19–20
116KDa I Reverse	5′-CCC TGC CAC CTG CCT CAG CTA-3′	11969–11949	21