Figure 7.

CCR8 expression during monocyte maturation in vitro and in response to monocyte-activating stimuli by immunofluorescence. Monocytes were obtained from three healthy donors by density centrifugation and adherence to serum-coated flasks. CCR8 expression was examined by quantitative immunofluorescence microscopy. CCR8 immunoreactivity is shown in green. Cells were counterstained with DAPI (blue). Depicted are monocytes after 2 hours of initial adherence (a), after 24 hours without any stimulation (b), and after 24 hours of stimulation with ZOP (c). Monocytes were allowed to further differentiate in culture to mature macrophages (d). Results of three individual experiments quantitating CCR8 expression are shown in the graph in e. Scale bar, 50 μm.