Figure 4.

A: Distribution of cell-cycle phases obtained from the cell-sorting analysis of RT4 and SCaBER. Cell-cycle-specific cytospins including cell-cycle subpopulations were obtained to evaluate the expression of KiSS-1 along cell cycle by in situ hybridization. B: Representative patterns of KiSS-1 in bladder cancer cell lines under study by in situ hybridization derived from a transitional superficial tumor (RT4) and a squamous bladder tumor (SCaBER). No differential expression of KiSS-1 by in situ hybridization was observed in these sorted cells spotted on cytospins containing enriched populations of each cell cycle phase. Original magnifications, ×200.