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Phosphorylation of STAT3 in the LNCaP-IL-6− and LNCaP-IL-6+ sublines. LNCaP sublines were treated with IL-6 for 30 minutes (lane 2, 10 ng/ml) and the presence of pSTAT3 and total STAT3 was investigated in nuclear extracts with monoclonal antibodies by Western blot. Lane 1, Untreated cells.
