Table 1.
Antibodies
| Antigen | Antibody | Clone |
|---|---|---|
| Vimentin | Mouse anti-human monoclonal | V9; Kappa Sigma, cat. no. V6630 |
| Desmin: | Mouse anti-desmin monoclonal | D33; DAKO, cat. no. M0724 |
| Smooth muscle α-actin | Mouse anti-human monoclonal | Clone 1A 4; Sigma, cat. no. A2547 |
| β-actin | Rabbit affinity-isolated | Sigma, cat. no. A2066 |
| Smooth muscle myosin heavy chain | Mouse anti-human monoclonal | Clone hSM-V; Sigma cat. no., M7786 |
| h-caldesmon | Mouse anti-human monoclonal | Clone hHCD; Sigma cat. no., C4562 |
| Smoothelin* | Mouse anti-human monoclonal | R4 |
| ID1 | Rabbit polyclonal IgG | C-20; Santa Cruz Biotech., cat. no. sc-488 |
| ID2 | Rabbit polyclonal IgG | C-20; Santa Cruz Biotech., cat. no. sc-489 |
| ID3 | Rabbit polyclonal IgG | C-20; Santa Cruz Biotech., cat. no. sc-490 |
| Negative control | Mouse IgG1 Kappa | MOPC21; Sigma, cat. no. I5381 |
*A kind gift from Dr. Guillaume van Eys, University of Masstricht, The Netherlands. 21