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. 2003 Feb;162(2):381–389. doi: 10.1016/S0002-9440(10)63833-3

Table 2.

Synthetic Oligonucleotides Used for Hybridization AT Tailing (HybrAT) Probes

Probe Position Sequence
AS-5′ biotin-SIVnef(AT)10 9380–9419* 5′ biotin-CCCATAAGTCTCCCCACGCGCCCGCAAGAGTCTCTGTCGCATATATATATATATATATAT-3′
S-5′ biotin-SIVnef(AT)10 9419–9380 5′ biotin-GCGACAGAGACTCTTGCGGGCGCGTGGGGAGACTTATGGGATATATATATATATATATAT-3′
AS-5′ biotin-HIVnef(AT)10 8910–8871 5′ biotin-GCTCCATGTTTTTCCAGGTCTCGGGATGCTGCTCCCACCCCATATATATATATATATATAT-3′
S-5′ biotin-HIVnef(AT)10 8871–8910 5′ biotin-GGGGTGGGAGCAGCATCCCGAGACCTGGAAAAACATGGAGCATATATATATATATATATAT-3′
AS-5′ biotin-HIVgag1(AT)10 1484–1447 5′ biotin-CTTGGTTCCTCATCTGGCCTGGTGCAATAGGCCCTGCATATATATATATATATATAT-3′
S-5′ biotin-HIVgag1(AT)10 1447–1484 5′ biotin-GCAGGGCCTATTGCACCAGGCCAGATGAGAGAACCAAGATATATATATATATATATAT-3′
AS-5′ biotin-HIVgag2(AT)10 1654–1617 5′ biotin-GTCCTTGTCTTATGTCCAGAATGCTGGTAGGGCTATACATATATATATATATATATAT-3′
S-5′ biotin-HIVgag2(AT)10 1617–1654 5′ biotin-GTATAGCCCTACCAGCATTCTGGACATAAGACAAGGACATATATATATATATATATAT-3′
AS-5′ biotin-HIVgag3(AT)10 1864–1827 5′ biotin-TATGGCCGGGTCCTCCCACTCCCTGACATGCTGTCATCATATATATATATATATATAT-3′
S-5′ biotin-HIVgag3(AT)10 1827–1864 5′ biotin-GATGACAGCATGTCAGGGAGTGGGAGGACCCGGCCATAATATATATATATATATATAT-3′

*Nucleotide positions refer to the SIVmac239 strain 20 (GeneBank, M33262).

Nucleotide positions refer to pNL4-3 21 (GeneBank, M19921).

AS is anti-sense orientation and S is sense orientation.