Figure 2.

The endogenous B56γ1 and γ2 isoforms localize to the cis-Golgi area. F10 cells were mildly permeabilized so that the Abs would not penetrate into the nucleus and detect nucleus-localized B56γ subunits. The cells were then double labeled with the anti-B56γ1/2 Ab and either the anti-GM130 (A) or the anti-p115 Ab (B). The anti-B56γ1/2 Ab was stained with Cy5 (red, left) and the other two Abs with Cy2 (green, middle). Cy5 and Cy2 images were then merged (right).