Figure 4.
Scatchard analysis of the competitive displacement of 125I-RTf by unlabeled RTf by the HSC transferrin receptor. A: HSC were incubated with 0.5 μg/ml 125I-RTf ± increasing concentrations of unlabelled RTf at 37°C for 2 hours. B: Scatchard analysis indicated a single class of binding sites for RTf with an estimated Kd of 5.08 ± 2.96 × 10−8 and a Bmax of 6.25 ± 3.56 × 10−11, with approximately 18,600 receptor sites per cell. Results are expressed as means from duplicate determinations in each experiment from HSC isolated from two separate rats.