Table 2.
Accumulation of T-Helper Cell Subsets on Unstimulated MLEC and MHEC Monolayers under Defined Flow in Vitro
Shear stress (dynes/cm2) | Th1 (cells/mm2) | Th2 (cells/mm2) | |
---|---|---|---|
MLEC (n = 3) | 0.5 | 301 ± 181 | 241 ± 140 |
0.7 | 230 ± 141 | 136 ± 39 | |
1.0 | 26 ± 6 | 10 ± 6 | |
MHEC (n = 5) | 0.5 | 501 ± 103 | 263 ± 40 |
0.7 | 307 ± 81 | 129 ± 35 | |
1.0 | 85 ± 48 | 41 ± 13 |
Th1 and Th2 cells at 1.0 × 106/ml were drawn across unstimulated MLECs or MHECs at the indicated shear stress. The total number of interacting cells (ie, rolling and arrested) from four different fields was determined after a minute of perfusion at each level of shear stress. No Th1 or Th2 transmigrations of MLEC or MHEC monolayers were observed.