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. 2003 May;162(5):1591–1601. doi: 10.1016/S0002-9440(10)64293-9

Table 2.

Accumulation of T-Helper Cell Subsets on Unstimulated MLEC and MHEC Monolayers under Defined Flow in Vitro

Shear stress (dynes/cm2) Th1 (cells/mm2) Th2 (cells/mm2)
MLEC (n = 3) 0.5 301 ± 181 241 ± 140
0.7 230 ± 141 136 ± 39
1.0 26 ± 6 10 ± 6
MHEC (n = 5) 0.5 501 ± 103 263 ± 40
0.7 307 ± 81 129 ± 35
1.0 85 ± 48 41 ± 13

Th1 and Th2 cells at 1.0 × 106/ml were drawn across unstimulated MLECs or MHECs at the indicated shear stress. The total number of interacting cells (ie, rolling and arrested) from four different fields was determined after a minute of perfusion at each level of shear stress. No Th1 or Th2 transmigrations of MLEC or MHEC monolayers were observed.

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