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. 2003 May;162(5):1521–1528. doi: 10.1016/S0002-9440(10)64285-X

Figure 1.

Figure 1.

ANGPTL4 is a direct target of hypoxia in HMEC-1 cells. a: Northern blot analysis of ANGPTL4 mRNA induction by chemical hypoxia (100 μmol/L DFO) and gaseous hypoxia (3% O2). b: Kinetic analysis by real-time quantitative reverse transcriptase-polymerase chain reaction of ANGPTL4 mRNA induction by 100 μmol/L of DFO. c: Northern blot analysis of ANGPTL4 mRNA levels after 15 hours of treatment with various growth factors and 100 μmol/L of DFO, demonstrating the specificity of the effects of hypoxia. d: Northern blot analysis of ANGPTL4 mRNA levels after incubation for 15 hours in an atmosphere containing 3% O2, with or without 1 μg/ml of actinomycin D. e: Northern blot analysis of ANGPTL4 mRNA levels after incubation for 4 hours in an atmosphere containing 3% O2, with or without 10 μg/ml of cycloheximide. f: Western blot analysis of ANGPTL4 protein, secreted into the culture medium in response to hypoxia. Recombinant ANGPTL4 protein secreted by stably transfected CHO cells was used as a control (right lane).