Table 1.
Extracellular Matrix-Associated Genes Up-Regulated in Th1 (T1)- and Th2 (T2)-Type Fibroblasts Compared with Untreated Normal (N) Fibroblasts
| Gene name | T1 versus N | T2 versus N | N (PE versus media) | T1 (PE versus media) | T2 (PE versus media) |
|---|---|---|---|---|---|
| Collagen 1a1 | 1.6 | 2.3 | 2.6 | 2.8 | 1.5 |
| Fibronectin | 0.6 | 0.9 | 3.0 | 2.2 | 1.3 |
| Collagenase-like A | Down | Down | 0.0 | 0.0 | Up |
| MMP-13 | 0.0 | 0.0 | Up | Up | Up |
| Thrombospondin 1 | 1.7 | 0.3 | 6.5 | 0.0 | 0.1 |
| uPA | Up | 0.0 | 1.0 | 1.9 | Up |
| PAI-1 | 0.6 | Down | 1.2 | 1.0 | 45.6 |
| tPA | 0.7 | 0.2 | 1.0 | 1.5 | 3.2 |
| MMP-3 (stromlysin) | 0.0 | 0.0 | Up | 0.0 | Up |
| MMP-7 (matrilysin) | 0.0 | 0.1 | 0.5 | Up | 56.3 |
| TIMP-1 | 2.3 | 0.5 | 1.2 | 1.3 | 2.0 |
| TIMP-2 | 1.3 | 0.2 | 1.7 | 0.6 | 5.5 |
| Hyaluronidases | Up | Up | Up | 7.8 | Down |
All three fibroblast cell lines were also analyzed in the presence and absence of IL13-PE (PE versus media) treatment. Data shown indicate fold induction in gene expression compared with normal fibroblasts (column 1 and 2) or compared with untreated fibroblasts cell lines (columns 3 to 5).