Proinflammatory cytokine gene expression in steatotic OLTs. TNF-α and IFN-γ were highly expressed in control OLTs and markedly depressed in CS1-treated liver grafts at day 1 (lanes 2 and 3, respectively), day 3 (lanes 4 and 5, respectively), and day 7 (lanes 6 and 7, respectively). Indeed the levels of these cytokines in the CS1-treated livers were comparable to those from nave livers (lane 1). Bottom: The ratios of TNF-α/β-actin (day 1, 0.23 ± 0.1 versus 0, P < 0.04; day 7, 0.41 ± 0.03 versus 0.01 ± 0.01, P < 0.001) and IFN-γ/β-actin (day 1, 1.4 ± 0.1 versus 0.8 ± 0.2, P < 0.04; day 7, 3.3 ± 0.4 versus 0.8 ± 1, P < 0.04) in control and CS1-treated OLTs, respectively (n = 3/group).