Table 1.
Immunohistochemical Markers of Malgun Cells
| Antibody | Source | Description/clone | Dilution | Staining | ||
|---|---|---|---|---|---|---|
| Pattern | Intensity* | Frequency (%)† | ||||
| CK8 | Dako‡ | Monoclonal, 35βH11 | 1:50 | C-diffuse | +++ | 100 |
| PARP | Santa Cruz | Monoclonal, Sc-8007 | 1:100 | N-diffuse | +∼++ | 80 ± 15 |
| Ku70 | Santa Cruz | Goat antiserum, Sc-1487 | 1:100 | N-diffuse | +++ | 92 ± 5 |
| Ku80 | Santa Cruz | Goat antiserum, Sc-1484 | 1:200 | N-diffuse | +∼++ | 85 ± 6 |
| OGG1 | Novus | Rabbit antiserum, nb100-106 | 1:80 | N-diffuse | +++ | 75 ± 15 |
| PCNA | Dako | Monoclonal, PC10 | 1:100 | N-diffuse | +∼+++ | 90 ± 5 |
| Ki67 | Dako | Rabbit antiserum, A0047 | 1:200 | N-speckled | +++ | 40 ± 12 |
| iNOS | Santa Cruz | Rabbit antiserum, Sc-651 | 1:200 | N-diffuse | +∼++ | 85 ± 10 |
| MSH2 | Pharmingen | Monoclonal, 6505A | 1:250 | N-diffuse | +∼+++ | 55 ± 8 |
| P53 | Dako | Monoclonal, M887 | 1:1600 | N-diffuse | +++ | Rare |
| Bcl2 | Dako | Monoclonal, M7001 | 1:40 | N-diffuse | +++ | Rare |
| CK: high mol wt | Dako | 35β E12 | 1:50 | − | − | 0 |
| Synaptophysin | Signet | Monoclonal, SY38 | 1:80 | − | − | 0 |
| Chromogranin A | Dako | Monoclonal, DAK-A3 | 1:200 | − | − | 0 |
| Neurofilament | Dako | Monoclonal, 2F11 | 1:100 | − | − | 0 |
| COX-2 | Santa Cruz | Rabbit, Sc-1746 | 1:100 | − | − | 0 |
N, nucleus; C, cytoplasm.
*Graded from negative (−) to very strong (+++).
†The average percentage and range of positive cells were calculated from three different biopsies by counting total 200 malgun cells, respectively.
‡DAKO, Carpinteria, CA; Santa Cruz, Santa Cruz, CA; Novus, Littleton, CO; Pharmingen, San Diego, CA; Signet, North Yorkshire, United Kingdom.