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. 2003 Apr;162(4):1323–1338. doi: 10.1016/S0002-9440(10)63928-4

Figure 6.

Figure 6.

E-cadherin promoter activity was decreased in iron-loaded primary rat hepatocyte cultures. Primary rat hepatocytes were maintained for 7 days in control medium, after which, cultures were maintained in either control medium or medium supplemented with 20 μmol/L of TMH-ferrocene. After 12 days of treatment, control and TMH-ferrocene-treated (+TMHF) cultures were transfected with either: CMV-renilla plasmid alone (control); CMV-renilla and pGL3 plasmids (pGL3); or CMV-renilla and pGL3/PECAD plasmids. At 24 hours after transfection, cultures were harvested and analyzed for relative (firefly/renilla) luciferase activity (RLU).