Figure 7.

Immunolocalization of paracellular junction proteins after iron loading of primary rat hepatocytes. Primary rat hepatocytes were maintained for 7 days in control medium, after which, cultures were maintained in either control medium or medium supplemented with 20 μmol/L of TMH-ferrocene for 21 days. After 21 days of iron loading, cultures were fixed and immunofluorescence was performed for E-cadherin (A and B), occludin (C and D), and ZO-1 (E and F) on control (control) and iron-loaded (+TMHF) cultures. (A, inset) No immunofluorescence was observed when cultures were incubated in control mouse IgG antibody rather than primary antibody. Original magnifications, ×600.