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A: Immunohistochemical staining of nuclear p16 protein in normal F344 (left) and F344 to LEW allograft at day 60 (middle). No nuclear staining is observed in the absence of the primary antibody (right). (×400) B: Densitometry of total p16 staining on Western blot of F344 and LEW renal allografts on various time points after transplantation, corrected for actin content of the samples. Data are expressed as mean + SEM (sem). (n = 3 samples for all groups).
