A: Senescence-associated β-galactosidase staining (SA β-gal) (pH 6.0) in frozen sections of normal F344 kidney, F344 to LEW renal allograft at day 60, and a LEW to F344 renal allograft at day 60. Biopsies are representative for 6 biopsies in each group. (×250) B: Quantification of SA β-gal staining using Zeiss ks400 analysis for normal F344 kidneys, F344 and LEW renal allografts on day 60 post-Tx. Data are expressed as mean + SEM (sem) (n = 6 for all groups). C: Quantification of SA β-gal staining using Zeiss ks400 analysis. F344 to LEW day 7 (n = 3), day 14 (n = 3), day 30 (n = 3), day 60 (n = 6) and day 100 (n = 3), data expressed as mean + SEM (sem).