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. 2003 Apr;162(4):1313–1321. doi: 10.1016/S0002-9440(10)63927-2

Figure 1.

Figure 1.

Fn14 mRNA and protein expression in glioma cell lines cultured on tissue culture plastic or glioma-derived substrate. A: Northern blot analysis using RNA from each of the indicated cell lines cultured on tissue culture plastic (P) or glioma-derived ECM (M) was performed using the indicated cDNA probes. GAPDH was used as loading control and relative Fn14 mRNA expression was normalized to GAPDH and presented as fold induction over G112 cells cultured on tissue culture plastic. B: Immunoblot analysis of Fn14 expression in glioma cell lines. Total cell lysates were prepared from the indicated cell lines and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis using Fn14 polyclonal antiserum.