Figure 4.

Fas-mediated T lymphocyte killing induced by bladder TCC cell lines. Primary culture TCC cell lines, as effector, pretreated with (closed bars) or without (open bars) 0.75 μg/ml of the anti-FasL-neutralizing NOK-1, were co-cultured with lymphocytes as target cells for 18 hours. a: A Fas-positive Jurkat cell line was killed by the 1207 and 1512 FasL-positive bladder TCC cells but not by the RT112 FasL-negative bladder TCC cell line. b: Kinetics of Jurkat cell killing mediated by bladder TCC cell lines. c: PHA lymphoblasts were killed by autologous and allogeneic FasL-positive TCC cell lines. d: MLTC-derived T lymphocytes were killed by the autologous FasL-positive TCC cell line. All experiments were performed at a 10:1 E:T ratio. Target cell killing was measured by MTT assay and was expressed as relative to that of target cells with medium alone, set arbitrarily at 100%. The mean ±SD of triplicate co-culture is indicated.