Figure 2.
Unfixed, crude amyloidotic spleen homogenates from three patients with generalized AA amyloidosis were homogenized and separated by gel electrophoresis. Western blotting with an antibody directed against human AA amyloid identified bands of ∼6 and 12 kd. Immunolabeling with anti-AGE and anti-CML showed multiple bands ranging from 12 to >60 kd. Molecular weight standards in kd (MW). CB, Coomassie blue staining; Pt.-No., patient number; AA, Western blotting with anti-AA amyloid; AGE, anti-AGE; and CML, anti-CML.
