Table 3.

AA Amyloidosis Was Induced in BALB/c Mice Using Daily Injections of 7% Azocasein

Number of injections	CML serum (ng/mg protein)	CML spleen (ng/mg protein)	Copies RAGE mRNA/copies β-actin mRNA × 105
0 (n = 5)	8.25 ± 2.87	10.03 ± 3.14	31.14 ± 65.33
5 (n = 5)	6.65 ± 1.82	9.17 ± 2.70	1.91 ± 1.10†
10 (n = 5)	7.83 ± 2.29	8.13 ± 3.31	10.25 ± 5.80†
15 (n = 5)	4.86 ± 2.21*	9.35 ± 2.38	60.06 ± 46.98†
20 (n = 5)	8.84 ± 2.10*	9.14 ± 1.27	52.86 ± 57.88

*Fifteen versus 20 injections; P < 0.05.

^†Five versus 10 injections; P < 0.01; 5 versus 20 injections; P < 0.05.

Levels of CML in serum and spleen homogenates as well as the relative amount of mRNA of the RAGE were measured as indicated.