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Normal, ulcerative colitis (UC), and Crohn’s disease (CD) intestinal myofibroblasts express mRNA transcripts for MMP-1, MMP-2, and MMP-3 (lanes 2 to 4, respectively) and TIMP-1 and TIMP-2 (lanes 6 and 7, respectively). No MMP-9 mRNA was detected in any of the myofibroblasts (lane 5). Specificity of the PCR products was confirmed by DNA sequence analysis. One hundred-bp markers are shown in the left lane of the figure. The figure shown is representative of six separate experiments.
