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NAD–nicotinamide exchange reactions with CobB, HST2, or SIR2. (A) Reactions were performed with (lanes 1, 3, 5, and 7) and without (lanes 2, 4, 6, and 8) histones, as indicated. (B) Reactions with acetylated (lanes 1–3, 7–9) or unacetylated (lanes 4–6, 10–12) H4 or H3 peptides. Peptides were acetylated as described in Materials and Methods.
