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Deacetylation of an H4 peptide by HST2. The peptide was initially acetylated by HAT1. (A) H4 peptide deacetylation by HST2 depends on NAD. The cpm present in the peptide after enzyme incubation are depicted. (B) HPLC traces of the input unacetylated H4 peptide, the peptide after acetylation by HAT1, and the peptide after subsequent deacetylation by HST2.
