Skip to main content
. 2007 Mar 28;104(14):5812–5817. doi: 10.1073/pnas.0611505104

Fig. 1.

Fig. 1.

1-NA-PP1 inhibits Kin28as kinase activity in vivo. (A) Growth curves of Kin28as and isogenic WT strains in YPD with 2 μM 1-NA-PP1 or DMSO (solvent control) added 45 min after inoculation (arrow). (B) ChIP analysis of CTD-Ser-5 phosphorylation at ACT1 and PDR5 promoters. DNA immunoprecipitated with α-Ser-5 monoclonal antibody (H14) was quantitated and normalized by calculating fold enrichment over nontranscribed DNA in chromosome VI telomeric region. For each treatment, the normalized quantities for mutant strain were divided by normalized quantities for the respective isogenic WT strain. White bars represent this normalized ratio of Kin28ts3 grown at 25°C or 1 hour after shift to 37°C. Similarly, gray bars represent normalized ratio of Kin28as treated for 1 hour with 1-NA-PP1 or DMSO.