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. 2007 Mar 26;104(14):5818–5823. doi: 10.1073/pnas.0608864104

Fig. 2.

Fig. 2.

Proton inventory for RG cleavage. The relative kobs (normalized to 1 in 100% H2O) was measured in different mole fractions of D2O (nD2O), ranging from 0 (100% H2O) to 1 (99.9% D2O). Each point is the average of two to nine independent trials. Data were fit to equations for a one-proton [kn/k0 = (1 − n + nΦT)] or two-proton [kn/k0 = (1 − n + nΦT)2] inventory (solid and dashed lines, respectively) and assume equal transition state contributions for the two transfers. n is the fraction of D2O in the reaction, and ΦT is the transition state fractionation factor (30). Data fitting was done with SigmaPlot (Systat Software, Point Richmond, CA) by using nonlinear least squares and were weighted more toward trials with greater numbers of repeats.