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. 2007 Mar 28;104(14):5947–5952. doi: 10.1073/pnas.0700638104

Fig. 6.

Fig. 6.

A mutation in the PAPP domain of lin-23 shortens the extended lifespan of daf-2 mutants. (A and B) The lin-23(ot1) mutation shortens the extended lifespan of daf-2 mutants. (A) daf-2(e1368) (green curve): mean = 36.3 ± 1.1, n = 43/72; lin-23(ot1); daf-2(e1368) line #2 (bright red curve): mean = 21.6 ± 0.7, n = 46/68, P < 0.0001 vs. control; lin-23(ot1); daf-2(e1368) line #1 (deep red curve): mean = 23.7 ± 0.6, n = 56/86, P < 0.0001 vs. control. (B) daf-2(e1370) (green curve): mean = 55.2 ± 2.0, n = 58/86; lin-23(ot1); daf-2(e1370) line #1 (deep red curve): mean = 37.4 ± 2.2, n = 41/79, P < 0.0001 vs. control; lin-23(ot1); daf-2(e1370) line #2 (bright red curve): mean = 38.8 ± 1.6, n = 57/90, P < 0.0001 vs. control. In the above experiments, worms were grown at 20°C on normal food (OP50) for their entire life. (C) lin-23(ot1) mutants display significantly reduced lifespan extension in response to daf-2 RNAi as compared with wild-type worms. lin-23(ot1) mutants grown at 20°C on normal OP50 bacteria from hatching until L4 and then shifted to feeding RNAi bacteria expressing dsRNA for the control empty vector (bright red curve, mean = 19.1 ± 0.5, n = 62/106) and daf-2 (deep red curve, mean = 24.3 ± 1.5, n = 68/105, P < 0.0001 vs. control). N2 worms grown under similar conditions on control empty vector (bright green curve, mean = 20.7 ± 0.6, n = 58/111) and daf-2 RNAi (deep green curve, mean = 39.7 ± 1.4, n = 74/106, P < 0.0001 vs. control). Similar results were obtained when the worms were grown at 20°C during development and transferred to 25°C as adults, except with daf-2(e1370). lin-23(ot1) extended the lifespan of daf-2(e1370) mutants at 25°C (SI Table 6).