Table 2.

Hill parameters for the cAMP response

Strain	bcAMP, MU/hr	fcAMP	CcAMP, μM	mcAMP
TK250 (ΔcyaA ΔlacY)	9.1	91 ± 5	645 ± 43	2.1 ± 0.1
TK310(ΔcyaA ΔcpdA ΔlacY)	5.9	240 ± 13	320 ± 32	1.0 ± 0.1
Prediction based on thermodynamics	—	>20	10–1,000	1

The first two rows give the parameters derived from fit of cAMP response of cyaA mutants to the Hill function (Eq. 2). The last row gives the best estimates of these parameters according to the thermodynamic model and the known biochemical parameters (see SI Methods for details).