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. 2007 Jan 31;35(4):1222–1229. doi: 10.1093/nar/gkl1091

Figure 2.

Figure 2.

(a) Time-dependent primer extension using B-TTP and TTP: reaction was performed with 5 µM 5′5′-32P-primer/template, 0.4 mM of each dNTPs, 0.4 mM of B-TTP, and Klenow 0.04 units. Electrophoresis was conducted on 19% acrylamide gel. (b) Primer extension using B-TTP analyzed on 15% acrylamide gel: reaction was performed with 5 µM 5′5′-32P-primer/template, 0.4 mM of each dNTPs, 0.4 mM of B-TTP, and Klenow 0.04 units. From left to right, lane 1 (from left): M-TTP-DNA; lane 2: co-spot of M-TTP-DNA and TTP-DNA; lane 3: TTP-DNA; lane 4: co-spot of B-TTP-DNA, and TTP-DNA; lane 5 B-TTP-DNA, lane 6: primer.