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. 2007 Jan 31;35(4):1222–1229. doi: 10.1093/nar/gkl1091

Figure 4.

Figure 4.

Primer extension using the full-length DNA and boronic acid-labeled DNA as templates. Reaction was performed with 5 µM primer 1/template, 0.4 mM of each dNTPs, 0.4 mM of labeled-TTP (M-TTP and B-TTP), and Klenow 0.04 units for 1 h. After centrifugation–filtration, the reaction was performed with radio-labeled 5′-32P-primer 2 and 0.4 mM of each dNTPs. Co-spot 1: polymerization using M-TTP and TTP-derived DNA as templates, Co-spot 2: polymerization using B-TTP and TTP-derived DNA as templates. Primer 1: 5′-GCGTAATACGACTCACTATA-3′; Template DNA: 3′CGCATTATGCTGAGTGATATCCGTTGGACTACTCCGGCTT TCCGGCTTTGCATGT-5′; Primer 2: 5′-TGTACGTTTCGGCCTTTCGG-3′.