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. 2007 Jan 31;35(4):1222–1229. doi: 10.1093/nar/gkl1091

Figure 5.

Figure 5.

Primer extension using the full-length DNA and boronic acid-labeled DNA as templates. Each 50 µl reaction was performed with 1.2 µM primers 1 and 2/template, 0.25 mM of each dNTPs, 0.25 mM of labeled-TTP (B-TTP), and 3.5 units of high-fidelity DNA polymerase (Roche, Indianapolis, Ind.) under conditions of 1 cycle at 94°C for 2 min, 30 cycles at 94°C for 20 s, 59°C for 30 s, 72°C for 1 min and 1 cycle at 72°C for 7 min. Lane 1: Marker; lane 2: DNA synthesized using dNTPs; lane 3: DNA synthesized using B-TTP and the other three dNTPs. Primer 1: 5′-CGCCGCCCCCGCCGCG-3′ Primer 2: 5′-CGGCGGCCCGCGGGCG; Template DNA: 5′-CGCCGCCCCCGCCGCG-40N-CGCCCGCGGGCCGCCG-3′.