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. 2007 Jan 31;35(4):1230–1244. doi: 10.1093/nar/gkl1099

Figure 1.

Figure 1.

The pMV158-dso and its interactions with RepB. (A) Schematic representation (top) and nucleotide sequence (bottom) of the pMV158-dso. This region is divided into two loci, a bind locus containing the DDR, and a nic locus harbouring the PDR (proximal direct repeats) and the IR-I element (L and R, left and right arms respectively) that generates the hairpin where the 5′-GpA-3′ dinucleotide cleaved by RepB would be placed in a ssDNA configuration. The three DNA fragments used in B are represented above the scheme. As a reference, some coordinates of the pMV158 sequence are indicated in parenthesis. The two repeats (I and II) constituting the PDR, and the three repeats (I, II and III) that compose the DDR are displayed. The A-T tract located between the PDR and the DDR is shown below the sequence. (B) Complexes between RepB and its DNA target. The protein was incubated with labelled DNA fragments containing the nic locus, the bind locus or the entire dso. RepB concentrations were 0 (-), 0.3, 0.4 and 0.5 µM for the reactions containing the nic or bind locus, and 0 (-), 0.3, 0.5, 0.7, 1.2, 1.5 and 1.7 µM for the entire-dso fragment. The different complexes are indicated.