Table 2.

Representative siRNAs whose activities can be predicted by the 3nt-periodicity.

A. Ten siRNAs that exhibit less stable guide strand 5′ end but show lower activity (less than 30).

Position#	Sequence of the passenger strand	p.s. 5′-end (kcal/mol)	g.s. 5′-end (kcal/mol)	g.s. − p.s. (kcal/mol)	Activity (relative)	Our prediction values
31	GGGGUGGUGCCCAUCCUGG	−14.42	−12.21	2.21	17.03	23.42
101	GCGAGGGCGAUGCCACCUA	−12.61	−10.01	2.6	28.52	49.06
104	AGGGCGAUGCCACCUACGG	−13.22	−10.69	2.53	24.66	33.73
166	GUGCCCUGGCCCACCCUCG	−13.43	−11.55	1.88	0.00	15.34
167	UGCCCUGGCCCACCCUCGU	−13.35	−10.03	3.32	0.01	26.90
168	GCCCUGGCCCACCCUCGUG	−14.42	−10.56	3.86	22.78	24.37
327	CCGCGCCGAGGUGAAGUUC	−13.1	−9.8	3.3	28.13	32.45
677	CCGCCGCCGGGAUCACUCU	−14	−9.75	4.25	26.93	34.99
682	GCCGGGAUCACUCUCGGCA	−14.7	−12.25	2.45	15.45	38.79
683	CCGGGAUCACUCUCGGCAU	−13.84	−10.89	2.95	25.13	45.64
B. Ten siRNAs that exhibit more stable guide strand 5′ end but show higher activity (more than 70).
80	AGUUCAGCGUGUCCGGCGA	−8.8	−12.49	−3.69	73.34	50.73
204	CGUGCAGUGCUUCAGCCGC	−11.83	−14.66	−2.83	79.54	37.74
427	GAGUACAACUACAACAGCC	−10.4	−13.07	−2.67	85.32	69.32
428	AGUACAACUACAACAGCCA	−9.09	−11.97	−2.88	81.16	79.11
429	GUACAACUACAACAGCCAC	−10.32	−13.23	−2.91	75.82	61.18
455	AUAUCAUGGCCGACAAGCA	−7.08	−9.64	−2.56	85.53	70.93
475	AAGAACGGCAUCAAGGUGA	−7.49	−11.06	−3.57	70.62	62.47
491	UGAACUUCAAGAUCCGCCA	−8.93	−12.25	−3.32	78.09	54.98
492	GAACUUCAAGAUCCGCCAC	−10	−13.23	−3.23	75.05	46.75
595	AACCACUACCUGAGCACCC	−9.74	−13.07	−3.33	79.56	45.68