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. 2007 Jan 30;35(4):1075–1084. doi: 10.1093/nar/gkl1140

Figure 1.

Figure 1.

Effect of expanded (GAA)n repeats on RNA synthesis in E. coli. (A) Structure of the p185Δ plasmid, carrying (GAA)n·(TTC)n repeats in different orientations. (B) Northern blot analysis of GAA-containing mRNAs from the wild-type E. coli cells. Total RNA was hybridized with a probe, corresponding to either the 5′ part of the cat gene (upper panel) or RNAI, corresponding to the p15A origin (lower panel). (C) Quantitative analysis of the above data. (D) Normalized amounts of GAA-containing mRNAs from cells treated with chloramphenicol. (E) Normalized amount of the GAA-containing mRNAs from the rne(ts) mutant strain upon its cultivation at the non-permissive temperature.