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. 2007 Jan 30;35(4):1209–1221. doi: 10.1093/nar/gkl1148

Figure 3.

Figure 3.

Electromobility shift competition assays with c-myc CRD RNA, β-globin RNA or MDR-1 RNA. About 350 nM of purified recombinant His6-tagged CRD-BP was incubated with 40 nM [32P] c-myc CRD RNA nts 1705–1886 (50 000 c.p.m) with or without the indicated molar excess of unlabeled competitor RNA. (A) 1–10 molar excess of unlabeled c-myc CRD RNA nts 1705–1886 (lanes 3–6), c-myc CRD RNA nts 1705–1792 (lanes 7–10), MDR-1 RNA nts 746–962 (lanes 11–14) and β-globin RNA nts 1–145 (lanes 15–18) were used. (B) 10–50 molar excess of unlabeled c-myc CRD RNA nts 1705–1886 (lanes 3 and 4), c-myc CRD RNA nts 1705–1792 (lanes 5 and 6), β-globin RNA nts 1–145 (lanes 7 and 8) and MDR-1 RNA nts 746–962 (lanes 9 and 10) were used.